Article from Lara Macheriotou published in "Ecology and Evolution"
Work by PhD student Lara Macheriotou and colleagues resulted in the first publication of the year 2019 for the Marine Biology Research Group. The article describes a methodological investigation of the utility of our internal curated nematode reference sequence database for the definition of species-delimiting Operational Taxonomic Unit (OTU) clustering thresholds by quantifying intra- and interspecific genetic differentiation with p-distance. The derived thresholds were subsequently tested on artificial communities as well as the performance of the clustering algorithms Uparse, Uclust_ref, the cluster-free Dada2 algorithm and the RDP taxonomic classifier given differing reference databases.
The article is publically available through Ecology and Evolution at https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4814